Value of human epididymis protein 4 in time resolved immunoassay for detection of gynecologic cancers
10.3969/j.issn.1006?5725.2017.05.032
- VernacularTitle:人附睾蛋白4时间分辨荧光免疫分析在妇科肿瘤诊断中的价值
- Author:
Yi ZHANG
;
Daozhen CHEN
;
Ke WANG
;
Fei XU
;
Jun ZHAO
;
Biao HUANG
;
Jue ZHANG
;
Jun FAN
;
Bin ZHOU
- Keywords:
Endometrial cancer;
Ovarian cancer;
Cervical cancer;
Human epididymis protein 4;
Time resolved immunoassay
- From:
The Journal of Practical Medicine
2017;33(5):793-796
- CountryChina
- Language:Chinese
-
Abstract:
Using Eu3+as a tracer,a sandwich?type assay was established. HE4 in serum specimens from 225 patients were detected by TRFIA. Serum levels of HE4 in pelvic tumors were quantitatively analyzed. From receiver operating characteristic (ROC) curves,the reference values was calculated for endometrial cancer,cervical cancer and ovarian cancer,respectively. Results The working range of serum HE4 for TRFIA was 10~10000 pmol/L with a sensitivity of 7.5 pmol/L. The method offered less interaction with CA125 and CA199,and also provided a better correlation with ECLA,while the CV of intra?assay was below 10%. HE4 levels were significantly higher in endometrial cancer,cervical cancer and ovarian cancer groups than in healthy volunteer group,while did not differ significantly between uterine fibroid group and healthy women group. As aging,the risk for developing pelvic tumors increased. The area of ROC for malignant tumors was all above 0.5 and the accurate interpretation of HE4 was 60 pmol/L. Conclusion A stable,precise HE4 TRFIA is well established,which is helpful in diagnosis of gynecologic cancers.
