A Study on Quality of the Precise Powder Decoction Pieces of Medicinal Flowers Lonicerae japonicae Flos
10.11842/wst.2017.01.013
- VernacularTitle:花类药材金银花精准煮散饮片的质量评价
- Author:
Zhiyao REN
;
Wen XU
;
Jing ZHANG
;
He SU
;
Linlin DONG
;
Jiang XU
;
Xiaohui QIU
;
Zhihai HUANG
- Keywords:
Lonicerae japonicae Flos;
precise powder decoction pieces;
DNA barcode;
fingerprint
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2017;19(1):89-94
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed at evaluating the quality of the precise powder decoction pieces (PPDP) of L.japonicae Flos (LJF) compared with the traditional commercial slices with chemical fingerprint methods and DNA molecular identification technology.Different specifications of PPDP were prepared,their dry extract contents were in contrast with that of commercial slices.The three batches of commercial slices were collected,and the content uniformity,fingerprint and similarity evaluation before and after the mixing and pulverization were studied by HPLC-DAD and DNA sequence alignment.As a result,the paste rate of PPDP was slightly higher than that of the traditional commercial slices.The dissolution of chlorogenic acid of PPDP was higher than that of the traditional commercial slices.RSD of inter-assay dissolutions of chlorogenic acid of commercial slices was 11.93％,which was reduced to 8.29％ after mixing and preparing into PPDP.The fingerprint showed that the slimilarity of the fringerprint of the mixed and powdered LJF was elevated with 7 common peaks.All the common peaks were increased at different levels.In conclusion,compared with traditional commercial slices of LJF,PPDP apparently improved the dissolution rate and the quality uniformity,indicating that the boiled powder of CRP obviously presented vantages in clinic.
