Quality Evaluation of the Decoctions of Platycladus cacumen Between the Precise Powder Decoction Pieces and Traditional Chinese Medical (TCM) Slices
10.11842/wst.2017.01.016
- VernacularTitle:侧柏叶煮散饮片煎煮质量评价
- Author:
Lu GONG
;
Junqi BAI
;
He SU
;
Peng ZHANG
;
Shuiming XIAO
;
Xiwen LI
;
Zhihai HUANG
;
Jiang XU
- Keywords:
Precise powder decoction pieces;
DNA barcode;
Platycladus cacumen;
uniformity;
chromatography fingerprints
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2017;19(1):108-113
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed at comparing the precise powder decoction pieces and market raw TCM slices of P.cacumen over the decocting quality.ITS2 sequence was adopted as a DNA barcode to identify P.cacumen.The chemical composition of the medicinal materials was characterized by HPLC fingerprints for the evaluation of the similarity of precise powder decoction pieces and market TCM slices.The concentrations of quercitrin were determined using UPLC,and the characteristic common peaks were identified.In addition,the extraction efficiency between the market TCM slices and the precise powder decoction pieces was also compared by standard decoction method.It was found that P.cacumen was accurately identified by ITS2 sequences.HPLC fingerprints showed that the extraction efficiency and similarity of the precise powder decoction pieces increased compared with the market TCM slices.However,the extraction yield rate of the precise powder decoction pieces was improved by 20％ increased in accordance with the standard decoction method,while the contents of the index component,quercitrin,presented rare increase and the decocting rates of the other chemical components little change in the study.In conclusion,it was indicated that precise powder decoction pieces improved the extraction efficiency and uniformity in comparison with TCM slices.
