Promotion of miR-200b promoter methylation by MMC induces fibroblast apoptosis
10.3969/j.issn.1006-5725.2017.06.007
- VernacularTitle:丝裂霉素C调控miR-200b启动子甲基化诱导成纤维细胞凋亡
- Author:
Shuguang WANG
;
Jingcheng WANG
;
Yu SUN
;
Lianqi YAN
;
Xiaolei LI
;
Jihang DAI
- Keywords:
Mitomycin C;
Methylation;
Scar adhesion;
Apoptosis
- From:
The Journal of Practical Medicine
2017;33(6):876-879
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of the role of mitomycin C(MMC)in regulating miR-200b expression and inducing fibroblasts apoptosis. Methods Fibroblasts cultured in vitro were treated with different concentrations of MMC for 5 min and continue culture for 24 h. The expression of miR-200b were analyzed by Real-time PCR. Cell apoptosis were observed using TUNEL staining. The expression of cleaved-PARP,Bax and Bcl-2 were detected by Western blot. The methylation level of miR-200b promoter were measured by BSP. Results After treated with MMC,The expression of miR-200b significantly downregulated.TUNEL Staining analysis demonstrated MMC could significantly induce human fibroblasts apoptosis. Western blot results showed cleaved-PARP,Bax increased and Bcl-2 decreased.The methylation ratio of miR-200b promotor increased and has a significant dose dependent. Conclusion MMC induced human fibroblasts apoptosis by promoting miR-200b promoter methylation.
