Mitochondrial ultrastructure and function changes in SH?SY5Y induced by manganese

VernacularTitle:锰对SH?SY5Y细胞线粒体超微结构及功能改变的研究
Author: Dongmei SONG ; Li CHEN ; Junxiang MA ; Yuanyuan ZHANG ; Shixuan ZHANG ; Caixia GUO ; Tian CHEN ; Lin TIAN ; Piye NIU
Keywords: Manganese; SH?SY5Y cell line; Mitochondria dysfunction
From: The Journal of Practical Medicine 2017;33(5):680-683
CountryChina
Language:Chinese
Abstract: Objective To explore the mechanism of SH?SY5Y mitochondrial dysfunction treated by manganese to find a new potential therapeutic target. Methods Transmission Electron Microscopy(TEM)to observe the morphology of mitochondria. Cell treated with 250μmol/L for periods of time(2 h, 4 h, 6 h)while mitochondrial membrane potential(MMP)and ROS can be detected by FCM and fluorescence microplate reader. Results After treating with MnCl2 in 6 h, TEM images showed early vacuoles, lamellar structures of SH?SY5Y cells. Then test the mitochondrial membrane potential and showed that MMP would be decreased gradually. Meanwhile, analysis showed that in comparison with control, treatment group had a higher ROS level respectively (P < 0.05). Conclusion MnCl2 can cause mitochondrial damage through a mechanism closely related to disrupt the MMP or generate abundant ROS.