Effect of miRNA-143 on proliferation, migration and invasion of CNE-2Z cells of human nasopharyngeal cancer
10.19405/j.cnki.issn1000-1492.2017.04.004
- VernacularTitle:miRNA-143对人鼻咽癌细胞CNE-2Z增殖、迁移和侵袭的影响
- Author:
Xin ZHANG
;
Meijuan ZHENG
;
Yuanhong XU
- Keywords:
nasopharyngeal cancer;
miR-143;
CNE-2Z;
migration;
invasion
- From:
Acta Universitatis Medicinalis Anhui
2017;52(4):480-484
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the effects of miR-143 on proliferation, migration and invasion of human nasopharyngeal cancer CNE-2Z cells.Methods The expression of miR-143 in NP69 cells, CNE-1 cells and CNE-2Z cells were detected by using real-time PCR.The overexpression of miR-143 in CNE-2Z cells was constructed through infecting lentivrius, and the level of miR-143 was determined by using real-time PCR.Cell viability was detected by using a CCK-8 kit according to the manufacturer's instruction at indicated time points.The effectiveness of overex-pression of miR-143 on migration and invasion of CNE-2Z cells were detected by using Transwell cell migration and invasion assay.Results The expression level of miR-143 in CNE-2Z was significantly lower than those in NP69 and CNE-1 (P<0.01).The miR-143 over-expressed CNE-2Z cell was successfully established.The cell viability in CNE-2Z/ miR-143 group was significantly decreased compared with CNE-2Z and CNE-2Z/miR-NC (P<0.01).Overexpression of miR-143 inhibited cell migration and invasion of CNE-2Z cells significantly(P<0.01).Conclusion miR-143 might inhibit cell proliferation, migration and invasion in human nasopharyngeal cancer CNE-2Z cells, indicating its important role in diagnosis, treatment and prognosis of nasopharyngeal cancer.
