Evaluation of matrix-assisted laser desorption/ionisation time-of-flight mass spectrometryfor identification of clinical yeasts
10.19405/j.cnki.issn1000-1492.2017.04.008
- VernacularTitle:MALDI-TOF MS鉴定酵母菌应用评价
- Author:
Boyun ZHANG
;
Naifang YE
;
Zhongxin WANG
- Keywords:
yeast;
ITS gene sequencing;
MALDI-TOF MS
- From:
Acta Universitatis Medicinalis Anhui
2017;52(4):501-504
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the ability of MALDI-TOF MS in identification of the yeast isolated from clinical specimens.Methods 387 strains of yeasts were tested.All the strains were from clinical specimen and identified by VITEK MS and API ID 32C system at the same time.Sequencing of the internal transcribed spacer (ITS) regions of ribosomal DNA was used as the reference method in the analysis of a total of 387 yeast isolates.The accuracy of detected results was analyzed by the X2 test.Results The identification accuracy rates of VITEK MS, API ID 32C were respectively 97.7% and 93.0%.The difference was statistically significant (X2=9.439, P=0.002).There was no statistical significance between MALDI-TOF and ITS sequencing.Conclusion MALDI-TOF is a rapid simple and accurate method which is identified to species level in identification of yeasts.
