Role of Notch1 signal pathway in osteogenic differentiation of human periodontal ligament stem cells
10.11659/jjssx.01E017081
- VernacularTitle:Notch1信号通路对人牙周膜干细胞应力分化过程的调控
- Author:
Shuzuo ZHOU
;
Fan QIN
;
Jiaxi CUI
;
Li DONG
;
Jixiang ZHOU
- Keywords:
Notch1 signal pathway;
human periodontal ligament stem cells;
Jagged1;
DPAT;
dynamic tensile;
osteoblast differentiation
- From:
Journal of Regional Anatomy and Operative Surgery
2017;26(4):240-243
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Notch1 signal pathway on the osteogenic differentiation in human periodontal ligament stem cells(PDLSCs) under dynamic strain.Methods PDLSCs were separated from freshly extracted teeth then identified and prolifed.Notch1 signal pathway was regulated by chemicals.Dynamic strains were applied to PDLSCs with the tension plus system.Then Notch1 signal pathway key factor Notch intracellular domain(NICD),osteoblastic related indexes alkaline phosphatase(ALP) and bone morphogenetic proteins 2 (BMP2) were detected by western blot expression.The deformation rate of stress parameters was 0 to 12%,and the frequency was 0.1 Hz.The loading time was 0 h,6 h,12 h and 24 h.Results As Notch1 signal pathway was activated,the expression of osteogenic markers ALP and BMP2 both reduced (P<0.05).On the contrary, the expression of osteogenic markers ALP and BMP2 both increased obviously (P<0.05) as Notch1 signal pathway was inhibited.Conclusion The activated Notch1 signal pathway will inhibit osteogenic differentiation of PDLSCs under dynamic tensile.
