Cytotoxicity of ophiopogonin D′ for rat H9c2 cardiomyocytes
10.3867/j.issn.1000-3002.2017.04.005
- VernacularTitle:麦冬皂苷D′对大鼠心肌细胞H9c2的细胞毒性
- Author:
Sijia REN
;
Huanhua XU
;
Ming LI
;
Feiran HAO
;
Zengchun MA
;
Xianglin TANG
;
Qiande LIANG
;
Hongling TAN
;
Chengrong XIAO
;
Yuguang WANG
;
Yue GAO
- Keywords:
ophiopogonin D′;
H9c2 cells;
toxic actions
- From:
Chinese Journal of Pharmacology and Toxicology
2017;31(4):325-331
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the cardiotoxicity of ophiopogonin D′(OPD′) for rat H9c2 cardio? myocytes. METHODS H9c2 cells were exposed to OPD′ 0.1, 1, 5, 10, 20, 25 and 50 μmol·L-1 for 24 h. Cell viability was examined by MTS assay, and the morphological changes in H9c2 cells were quanti? fied. The cell nucleus injury was examined by high content immune fluorescence screening and the morphological changes were observed under a fluorescence microscope. After treatment with OPD′ 0.1, 1, 5 and 10 μmol·L- 1 for 24 h, the effect on reactive oxygen species (ROS), mitochondrial mem? brane potential(MMP) and apoptosis was detected by flow cytometry. RESULTS The viability was sig? nificantly reduced following exposure to OPD′ 0.1, 1, 5, 10, 20, 25 and 50 μmol·L- 1 (P<0.05,P<0.01). The IC50 value was 9.9 μmol ·L- 1 and cell shrinkage and apoptosis occurred. The levels of ROS and apoptosis rate of H9c2 cells were significantly increased after exposure to OPD′ 0.1, 1, 5 and 10 μmol·L-1 for 24 h (P<0.05,P<0.01) and MMP markedly declined (P<0.05,P<0.01). CONCLUSION OPD′ has significent cytotoxicity on H9c2 cells. It may be related to inducing apopotsis pathways.
