Effect of MKK34 on airway β-catenin in a HDM-induced mouse asthma
10.3969/j.issn.1006-5725.2017.04.011
- VernacularTitle:MKK34多肽对HDM哮喘小鼠气道β-catenin表达及分布的影响
- Author:
Yahui HU
;
Hangming DONG
;
Laiyu LIU
;
Chaowen HUANG
;
Lishan LUO
;
Xuan WAN
;
Haijin ZHAO
;
Shaoxi CAI
- Keywords:
MKK34;
HDM-induced mouse asthma;
β-catenin;
ERK pathway
- From:
The Journal of Practical Medicine
2017;33(4):543-547
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of MKK34 (a peptide spanning a C-terminal α-helical region in TSLP) on airway inflammation and β-catenin of airway epithelium in a HDM-induced mouse asthma.Methods 32 male BALB/c mice were randomly divided into control,MKK34,asthma and MKK34 + HDM groups.The mice in the asthma group were exposed to HDM for five consecutive days and the MKK34 + HDM group was pretreated with MKK34 1 h prior to the HDM intranasally treated.After 8 weeks' treatment,animal lung function test and pathological staining were performed to evaluate the asthma situation,IL-4,IFN-γin bronchoalveolar lavage fluid and IgE in the serum were detected,immunohistochemistry and western blot were used to assess β-catenin and p-ERK,t-ERK levels.Results Airway reactivity,IL-4 and IgE in the asthma group were significantly higher than that in the control group.Treatment with MKK34 significantly decreased airway hyperresponsiveness,IL-4 and IgE.HE staining demonstrated the chronic bronchitic inflammation in the lungs of asthma group.β-catenin in the control group was distributed evenly at the cytomembrane of epithelial cells.In the asthma group,β-catenin was disordered in epithelial cells and its expression was decreased.Treatment with MKK34 ameliorated the damage of β-catenin and chronic bronchitic inflammation.The protein levels of p-ERK1/2 increased obviously in the asthma group.The pretreated group significantly decreased the expression of p-ERK1/2.Conclusions MKK34 can ameliorate the airway inflammation and the destruction of β-catenin of airway epithelium in a HDM-induced mouse asthma.The ERK pathway may play a role in this process.
