Fluorescence in situ hybridization based on a panel of probes for detection of common cytogenetic abnormalities in multiple myeloma
10.3969/j.issn.1006-5725.2017.04.034
- VernacularTitle:组合探针荧光原位杂交检测多发性骨髓瘤常见染色体异常
- Author:
Shaoqian CHEN
;
Jing CHENG
;
Xiaobing JIANG
;
Shihong ZHANG
- Keywords:
Multiple myeloma;
Panel of probes;
Fluorescence in situ hybridization;
Conventional cytogenetic analysis
- From:
The Journal of Practical Medicine
2017;33(4):626-629
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the advantages of plasma cell enrichment combined with fluorescence in situ hybridization (FISH) based on a panel of probes by the conventional cytogenetic (CC) analysis.Methods Fresh heparinized bone marrow samples were collected by bone marrow biopsy.Plasma cells were enriched in BM samples using a magnetic cell-sorting procedure to select CD138+ cells.The common chromosome abnormalities of MM were detected by FISH based on a panel of probes and CC analysis after short-term culture of the BM cells,in order to compare the differences between these two methods for the frequency of common cytogenetic abnormalities.Results 72 of 95 (75.8%) MM patients were found to carry clonal chromosome abnormalities by FISH.And RB 1 deletion was the highest at 44.2% (42/95) followed by CKS1B (1q21) amplification (42.1%).The frequencies of CDKN2C (1p32) deletion,TP53 deletion,IGH/CCND1 and IGH/FGFR3 were 8.4% (8/95),12.6% (12/95),14.7% (14/ 95) and 14.7% (14/95),respectively.IGH/MAF was negative.Thirty-two of 95 (33.7%) patients were found to carry clonal aberrations by CC analysis.The frequency of chromosome abnormalities detected by FISH was significantly higher than CC analysis (75.8% vs 33.7%,P =0.000).Conclusion Plasma cell enrichment combined with FISH based on a panel of probes can greatly increase the frequency of chromosome abnormalities,which provides cytogenetic basis for risk stratification and prognosis of MM patients.