miR-let-7d regulates lung cancer cell proliferation and invasion abilities through nuclear receptor PPARγ
10.3969/j.issn.1000-4718.2017.04.020
- VernacularTitle:miR-let-7d通过调控核受体PPARγ促进肺癌细胞的增殖和侵袭
- Author:
Jiateng ZHONG
;
Zhigang GUO
;
Wei SU
- Keywords:
Lung cancer;
MicroRNA-let-7d;
Peroxisome proliferator-activated receptors γ
- From:
Chinese Journal of Pathophysiology
2017;33(4):699-704
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the phenomenon that miR-let-7d regulates the proliferation and invasion abilities of the lung cancer cells through nuclear receptor peroxisome proliferator-activated receptors γ (PPARγ).METHODS: The relation between PPARγ and microRNA was analyzed by bioinformatics.The plasmid reporter assay was used to verify that PPARγ was the target of miR-let-7d.The lung cancer cell line with low expression of PPARγ was selected from different lung cancer cell lines by Western blot.The regulatory role of miR-let-7d in the lung cancer cells was determined by dual luciferase labeling and Western blot.The effect of miR-let-7d on the proliferation ability of lung cancer cells was detected by colony formation assay, the effect of miR-let-7d on the invasive ability of lung cancer cells was detected by Transwell invasion assay.RESULTS: The results of bioinformatic analysis showed that miR-let-7d regulated the expression of PPARγ, and the 3'UTR of PPARγ contained 2 functional miR-let-7d binding sites, indicating that PPARγ is a direct target of miR-let-7d.miR-let-7d was able to directly regulate the expression of PPARγ at mRNA and protein levels.Transfection of miR-let-7d inhibitor promoted the proliferation and invasion abilities of lung cancer cells by increasing the expression of PPARγ.CONCLUSION: miR-let-7d increases the expression of tumor suppressor PPARγ to inhibit the proliferation and invasive abilities of lung cancer cells.
