Effects of silencing and overexpression of CBX8 on proliferation and apoptosis of human glioma cells
10.3969/j.issn.1000-4718.2017.04.024
- VernacularTitle:CBX8过表达或沉默对人神经胶质瘤细胞增殖和凋亡的影响
- Author:
Baiping ZHANG
;
Shukai SUN
;
Dong JIA
- Keywords:
Glioma;
Chromodomain protein 8;
T98G cells;
U87MG cells
- From:
Chinese Journal of Pathophysiology
2017;33(4):723-729
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effects of chromodomain protein 8 (CBX8) on the proliferation and apoptosis of human glioma cells.METHODS: The expression of CBX8 in the tissues and cells was detected by Western blot and RT-qPCR.The overexpression (Flag-CBX8) and silencing (sh-CBX8) vectors of CBX8 were constructed and transfected into glioma T98G cells and U87MG cells.The cell proliferation was detected by MTT assay and BrdU staining.The cell apoptosis was analyzed by flow cytometry.The protein expression of Rb/E2F1 was detected by Western blot.RESULTS: Compared with normal brain tissues and astrocytes, the expression of CBX8 was increased in the glioma tissues and glioma cells.Overexpression of CBX8 promoted the cell proliferation, inhibited the cell apoptosis, and upregulated the protein levels of Rb/E2F1.On the contrary, silencing of CBX8 inhibited the cell proliferation, promoted the cell apoptosis, and decreased the protein levels of Rb/E2F1 in the T98G cells and U87MG cells.Moreover, the expression of cyclin D1 and Bcl-2/Bax ratio were reduced after transfection with sh-E2F1 in the T98G cells and U87MG cells.CONCLUSION: CBX8 may regulate the proliferation and apoptosis of glioma cells through Rb/E2F1 pathway.
