Inhibitive effect of BSP on Caspase dependent apoptosis of RAW264.7 cells
10.3969/j.issn.1006-5725.2017.04.002
- VernacularTitle:骨唾液酸蛋白对Caspase介导的RAW264.7细胞凋亡的抑制作用
- Author:
Yan ZHANG
;
Yu SHI
;
Sinian LIU
;
Yi LI
;
Chao HAN
;
Ling LI
- Keywords:
BSP;
RAW264.7;
Caspase;
Apoptosis
- From:
The Journal of Practical Medicine
2017;33(4):510-513
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the inhibitive effect of BSP on Caspase dependent apoptosis of preosteoclasts RAW264.7 cells by bone sialoprotein (BSP).Methods RAW264.7 cells were treated with the inhibitors of signaling pathway molecules and/or BSP.The activities of caspase3/8/9 in RAW264.7 cells were detected with caspase3/7/8/9 kits and the interaction between Caspase-8 and integrin αVβ3 was detected by Co-IP.Results The activities of Caspase3/8/9 in BSP-treated RAW264.7 cells were decreased and the apoptosis of RAW264.7 cells were inhibited significantly.In addition,there was a positive correlation between caspase-8 activity and caspase-3 activity.There was interaction between caspase-8 and integrin αVβ3.Conclusion The anti-apoptotic effect of BSP on RAW264.7 cells is mainly through the regulation of exogenous apoptosis pathway and the apoptosis induced by caspase-8 is inhibited by the binding of BSP and αvβ3.
