The effects of Leptin in proliferation of MCF-7 breast cancer cells
10.3969/j.issn.1006-5725.2017.04.003
- VernacularTitle:瘦素促进人乳腺癌细胞株MCF-7增殖的机制
- Author:
Wenhui LIU
;
Qiujin ZHANG
;
Xinyi ZHOU
;
Jie ZHANG
;
Naixiang LUO
- Keywords:
Breast neoplasms;
Leptin;
Rab-25;
C-myc;
Proliferation
- From:
The Journal of Practical Medicine
2017;33(4):514-517
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of leptin on proliferation of the breast carcinoma MCF-7 cells in terms of protein and nRNA of Rab-25 and C-myc.Methods MCF-7 cells were treated with leptin and normal saline (NS) as control.The 490 nm OD values of cells were read by MTT and the protein and mRNA expressions of Rab-25 and C-myc were measured by western blot and RT-PCR methods in two groups.Results Compared with control group,leptin can promote the proliferation of MCF-7 cells in a dose and time dependent manner within certain limit and up-regulate the protein expressions of Rab-25 and C-myc in the breast carcinoma MCF-7 cells (P ＜ 0.05).However,leptin also can up-regulate the mRNA expressions of Rab-25 but without significance (P =0.05).On the contrary,leptin can also up-regulate the mRNA expressions of C-myc (P ＜ 0.05).Conclusion Leptin promotes breast cancer cells proliferation,which may be related to up-regulate the protein and mRNA expressions of Rab-25 and C-myc.It could provide theoretical foundation and new target of gene therapy in the clinical treatment of breast cancer.
