Expression of SRPX2 in human endometrial carcinoma and its role in cell proliferation and apoptosis
10.3969/j.issn.1006-5725.2017.04.007
- VernacularTitle:SRPX2在子宫内膜癌中的表达及对HEC-1A细胞增殖凋亡的影响
- Author:
Li WANG
;
Yong ZHANG
- Keywords:
Endometrial carcinoma;
Sushi Repeat Containing Protein X Linked 2;
Expression;
Proliferation;
Apoptosis
- From:
The Journal of Practical Medicine
2017;33(4):529-532
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of Sushi Repeat Containing Protein X Linked 2 (SRPX2) in hunan endometrial carcinoma (EC) and its role in cell proliferation and apoptosis Methods Immunohistochemistry was applied to detect the expression of SRPX2 in EC and matched tumor-adjacent tissues (n =50).The correlation between SRPX2 expression and clinical features was analyzed by Pearson chi-square test.siRNA was used to down-regulate the expression of SRPX2 in HEC-1A cells.The effects of SRPX2 on cell proliferation and apoptosis were measured by CCK-8 assay and Flow cytometer,respectively.The FAK and Cyclin D1 expression were detected after silencing SRPX2 expression.Results The expression of SRPX2 was significantly up-regulated in EC tissues,and the high expression of SRPX2 was associated with the large tumor size (＞3 cm) and advanced FIGO stage (Ⅲ+Ⅳ stage).Silencing SRPX2 could inhibit cell proliferation,induce cell apoptosis and repress FAK and Cyclin D1 expression in HEC-1A cells.Conclusion The expression of SRPX2 is significantly higher in EC tissues,which is closely related with cell proliferation.Scilencing SRPX2 could achieve an anti-cancer role through inhibiting cell proliferation and inducing cell apoptosis by the down-regulation of SRPX2.
