Simultaneous determination of five constituents in Jiangzhi Huoxue Tablets by UPLC-MS/MS

VernacularTitle:UPLC-MS/MS法同时测定降脂活血片中5种成分
Author: Chenning ZHANG ; Lin CHEN ; Jiuyun YANG ; Guangyi YANG ; Na XU ; Weidong MA ; Jinbao WEI ; Yonghong ZHANG
Keywords: Jiangzhi Huoxue Tablets; stilbene glycoside; tanshinone Ⅱ A; emodin; ferulic acid; puerarin; UPLC-MS/MS
From: Chinese Traditional Patent Medicine 2017;39(4):741-744
CountryChina
Language:Chinese
Abstract: AIM To establish an ultra-performance liquid-chromatography tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous content determination of five constituents in Jiangzhi Huoxue Tablets (Polygoni multiflori Radix,Astragali Radix,Chuanxiong Rhizoma,etc.).METHODS The 50％ methanol extract of this drug was performed on a 40 ℃ thermostatic Restek UItra BiPh column (100 mm × 2.1 mm,5 μm),with the mobile phase comprising of acetonitrile (containing 0.1％ formic acid)-0.1％ formic acid flowing at 0.4 mL/min in a gradient elution manner.RESULTS Stilbene glycoside,tanshinone Ⅱ A,emodin,ferulic acid and puerarin showed good linear relationships within the ranges of 4.01-1 027,0.7-187,1.48-380,3.98-1 020 and4.285-1 097 ng/mL (r ＞0.994 0),whose average recoveries were 98.57％-101.0％ with the RSDs (n =6) of less than 4.79％.CONCLUSION This specific and sensitive method can be used for the quality control of Jiangzhi Huoxue Tablets.