High glucose-activated NFAT promotes podocyte apoptosis by down-regulation of PGC-1
10.3969/j.issn.1000-4718.2017.04.008
- VernacularTitle:高糖通过下调PGC-1α激活NFAT并促进足细胞凋亡
- Author:
Enping CHEN
;
Ligen DU
;
Yinwei WU
;
Cuimei YE
;
Quanfang LUO
- Keywords:
High glucose;
Podocytes;
Apoptosis;
Peroxisome proliferator-activated receptor γ coactivator-1α;
Nuclear factors of activated T-cells
- From:
Chinese Journal of Pathophysiology
2017;33(4):620-626
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore whether down-regulation of peroxisome proliferator-activated receptor γ coactivator (PGC)-1α induces podocyte apoptosis and its mechanism.METHODS:The podocytes were cultured under high glucose (HG) condition and the cell apoptosis was analyzed by flow cytometry.The methods of real-time PCR and Western blot were used to analyze the mRNA and protein expression of related molecules in the control, HG-treated or siRNA-treated podocytes.RESULTS:The expression PGC-1α at mRNA and protein levels was significantly decreased in HG-injured podocytes.Down-regulation of PGC-1α expression in vitro by siRNA resulted in podocyte apoptosis.The nuclear protein expression of nuclear factor of activated T-cells (NFAT) was significantly increased in HG injured podocytes, indicating the NFAT activation.Down-regulation of PGC-1α expression also decreased the nuclear protein expression of NFAT.Moreover, silencing of NFAT expression by siRNA significantly abolished PGC-1α deficiency-induced podocyte apoptosis.CONCLUSION: Down-regulation of PGC-1α induces podocyte apoptosis.NFAT mediates PGC-1α deficiency-induced podocyte apoptosis.
