Role of fibroblast growth factor 8 in process of dental epithelium-induced directional differentiation of human postnatal dental pulp stem cells into odontoblasts and pulp cells
10.3969/j.issn.1000-4718.2017.04.025
- VernacularTitle:FGF8辅助牙源性上皮诱导hDPSCs分化为成牙本质细胞及牙髓细胞
- Author:
Hao LIU
;
Jianping JIANG
;
Juanjuan ZHANG
;
Zhifang PAN
;
Mengjie LI
;
Zheng LIANG
;
Xiangyu ZHAO
;
Yan SUN
;
Xiaoying LIU
- Keywords:
Fibroblast growth factor 8;
Human postnatal dental pulp stem cells;
Odontoblasts
- From:
Chinese Journal of Pathophysiology
2017;33(4):730-734
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of fibroblast growth factor 8 (FGF8) on directional differentiation of human dental pulp stem cells (hDPSCs) into odontoblasts and pulp tissue.METHODS: hDPSCs were isolated and cultured, and identified with flow cytometry by detecting cell surface markers of hDPSCs.FGF8 at concentration of 50 μg/L was added into the mineralization fluid to induce the differentiation of the hDPSCs.The mRNA expression of dentin sialophosphoprotein (DSPP), alkaline phosphatase (ALP), bone sialoprotein (BSP) and core-binding factor alpha 1 (Cbfa-1) in differentiated cells was detected by real-time PCR.FGF8 and mouse E11.5 dental epithelium formed restructuring cell group with hDPSCs, and then the restructuring cell group was transplanted under renal capsule membrane in nude mice for tissue culture.DNA in situ hybridization was used to identify the sources of odontoblasts and pulp cells.RESULTS: The surface markers of CD29 and CD90 showed positive in isolated hDPSCs.FGF8 induced hDPSCs to form a distinct mineralization nodule, and the expression of dentin-specific proteins, DSPP, BSP and Cbfa-1, was increased.hDPSCs were induced to differentiate into odontoblasts and pulp cells by E11.5 dental epithelium and FGF8.CONCLUSION: FGF8 can assist dental epithelium to induce directional differetiation of hDPSCs into odontoblasts and pulp cells, and formation of dentin and dental pulp cavity structure.
