Effect of interleukin-1β on activity of vacuolar proton pump and intracellular acidosis in osteoclasts
10.3969/j.issn.1000-4718.2017.04.029
- VernacularTitle:白细胞介素1β对破骨细胞空泡型质子泵活性与胞内酸化的影响
- Author:
Weiping ZHU
;
Jing ZHENG
;
Yijie LI
;
Zhonghe LI
- Keywords:
Interleukin-1β;
Vacuolar proton pump;
Osteoclasts
- From:
Chinese Journal of Pathophysiology
2017;33(4):749-753
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of interleukin-1β (IL-1β) on the activity of vacuolar proton pump (V-ATPase) and intracellular pH value in the osteoclasts for exploring the mechanism of IL-1β to promote osteoclastic bone absorption.METHODS: The mature osteoclasts were attained by induction of bone marrow monocytes/macrophages.The osteoclasts were cultured with α-MEM and treated with different concentrations (0.1, 0.3 and 0.5 μg/L) of IL-1β.After cultured for 48 h, the mRNA and protein expression of V-ATPase was detected.The effect of IL-1β on intracellular pH value, activity of V-ATPase and the bone absorption abilities of osteoclasts were examined.RESULTS: The expression level and activity of V-ATPase were significantly increased and the intracellular pH value of the osteoclasts decreased after incubated with IL-1β for 48 h.Under the same culture condition, the bone absorption capacity of the osteoclasts was promoted.The enhanced bone absorption capability of the osteoclasts was accompanied by an increase in the concentration of IL-1β.CONCLUSION: The mechanism of IL-1β participating in pathological bone absorption in the bone and joint inflammatory diseases is that IL-1β increases the expression and activity of V-ATPase, boosts the production and/or transportation of hydrogen ion, leading to increased osteoclastic bone absorption activity, and resulting in bone destruction.
