Simultaneous determination of seven constituents in Sangqishouwu Tablets by HPLC
10.3969/j.issn.1001-1528.2017.04.015
- VernacularTitle:HPLC法同时测定桑芪首乌片中7种成分
- Author:
Zhengyan LI
;
Xiaolin YUAN
;
Danxia WEI
;
Yueting LI
;
Xiaoling YU
- Keywords:
Sangqishouwu Tablets;
chemical constituents;
HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(4):737-740
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of calycosin-7-glucoside,ononin,calycosin,formononetin,oxypaeoniflorin,alibiflorin and benzoylpaeoniflorin in Sangqishouwu Tablets (Talxilli Herba,Astragali Radix,Polygoni multiflori Radix Praeparata,etc.).METHODS The analysis of methanol extract of this drug was performed on a 25 ℃ thermostatic Diamonsil C18 colmn (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.02％ phosphoric acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 230 nm and 254 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ≥ 0.999 2),whose average recoveries were 97.13％-100.03％ with the RSDs of 0.69％-1.47％.CONCLUSION This sensitive,accurate and specific method can be used for the rapid quality control of Sangqishouwu Tablets.
