Effects of dexamethasone on MRL/Ipr mice with systemic lupus erythematosus complicated with cognitive dysfunction
10.11817/j.issn.1672-7347.2017.03.003
- VernacularTitle:地塞米松对MRL/Ipr狼疮小鼠认知功能的保护作用
- Author:
Yuanyuan WANG
;
Jie TANG
;
Lin SHEN
;
Jiangyan LI
;
Cheng ZHA
;
Rui WANG
;
Kun HU
;
Jin XI
;
Jianrong CHANG
;
Changhao XIE
- Keywords:
systemic lupus erythematosus;
dexamethasone;
cognitive function;
PI3K/Akt/NF-κB p65
- From:
Journal of Central South University(Medical Sciences)
2017;42(3):251-256
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of dexamethasone on systemic lupus erythematosus complicated with cognitive dysfunction.Methods:Ten wild type mice and 20 MRL/lpr mice were applied for the research.MRL/lpr mice were randomly assigned to a MRL/lpr group and a MRL/lpr + dexamethasone (1.5 mg/kg) group.Interleukin-6 (IL-6),IL-1β,and tumor necrosis factor alpha (TNF-α) in serum and hippocampus were detected.The protein phosphorylation levels of phosphoinositide 3-kinase (P-PI3K),protein kinase B (P-Akt),NF-kappa-B inhibitor alpha (P-IκBa) and nuclear transcription factor kappa-B p65 (P-NF-κB p65) were detected by Western blot,the level of P-NF-κB p65 also was detected by immunohistochemistry.Results:Treatment with dexamethasone (1.5 mg/kg) alleviated the cognitive dysfunction and decreased the levels of IL-6,IL-1 β and TNF-α in serum and hippocampus,and reduced the levels of P-PI3K,P-Akt,P-IκBa and P-NF-κB p65 in hippocampus in MRL/lpr mice.Conclusion:Dexamethasone may play a protective role in the cognitive function by decreasing the levels of TNF-α and IL-1 β in the hippocampus of MRL/lpr lupus mice.
