Simultaneous determination of four constituents in Canarii Fructus by RP-HPLC
10.3969/j.issn.1001-1528.2017.04.023
- VernacularTitle:RP-HPLC法同时测定青果中4种成分
- Author:
Xue HAN
;
Jinghua LU
- Keywords:
Canarii Fructus;
gallic acid;
protocatechuic acid;
chlorogenic acid;
epicatechin;
RP-HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(4):773-776
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a RP-HPLC method for the simultaneous content determination of four constituents in Canarii Fructus.METHODS The analysis of Canarii Fructus methanol extract was performed on a 30 ℃ thermostatic Hypersil ODS column (4.6 mm × 200 mm,5 μm),with the mobile phase comprising of acetonitrile0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 237 nm.RESULTS Gallic acid,protocatechuic acid,chlorogenic acid and epicatechin displayed good linear relationships within the ranges of 0.525-10.500 μg/mL (r =0.999 9),0.700-14.000 μg/mL (r =0.999 9),0.575-11.500 μg/mL (r =0.999 9) and 0.550-11.000 μg/mL (r =0.999 9),whose average recoveries were 99.05％ (RSD=1.4％),98.73％ (RSD=0.8％),98.69％ (RSD=1.3％) and 99.47％ (RSD=1.1％),respectively.CONCLUSION This simple,sensitive and relible method can be used for the quality control of Canarii Fructus.
