Correlation between the Three Single Nucleotide Polymorphisms in Angiotensin Ⅱ Type one Receptor Gene and Coronary Artery Disease
10.3969/j.issn.1671-7414.2017.02.015
- VernacularTitle:血管紧张素Ⅱ1型受体3个单核苷酸多态性与冠心病的相关性分析
- Author:
Yangdong ZHANG
;
Jianrong ZHOU
;
Ruixue CHU
;
Liying WANG
;
Liang FANG
- Keywords:
angiotensin Ⅱ type one receptor;
single nucleotide polymorphism;
coronary artery disease;
haplotype
- From:
Journal of Modern Laboratory Medicine
2017;32(2):57-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between the three single nucleotide polymorphisms (SNP) in angiotensin Ⅱ type one receptor (AT1R) gene with coronary artery disease (CAD) in Chinese Han nationality.Methods Extracted DNA and RNA samples of peripheral blood white cells from 192 CAD patients and 189 healthy individuals in Jan 2011 to Oct 2013 from the general hospital of the PLA Rocket Force.Designed primes and the three SNPs as rs6801836,rs2675511 and rs5182 of AT1R gene were analyzed with allele-specific fluorogenic oligonucleotide probes in an assay combining extension and hybridization.Results The genotype and allele frequencies of the three SNPs were not significantly different between the control group and CAD group (x2 =0.047～2.226,all P＞0.05).The major haplotype constructed with linkaged rs6801836 and rs5182 was TT.The frequency of every haplotypes showed no significantly difference between the two groups (x2 =0.025～1.020,all P＞0.05).Conclusion The three polymorphisms of AT1R gene studied in this work showed no association with CAD susceptibility.
