Biological characteristics of bacteriophage D218 of multiple drug resistance Acinetobacter baumanii
10.3969/j.issn.1673-4130.2017.07.004
- VernacularTitle:多耐药鲍曼不动杆菌噬菌体D218的生物学特性研究
- Author:
Chunming ZHANG
;
Bin XU
;
Jun XIANG
;
Jing GAO
- Keywords:
multiple drug-resistance acinetobacter baumanii;
bacteriophage;
infection treatment
- From:
International Journal of Laboratory Medicine
2017;38(7):876-879
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the bacteriophage for splitting multiple drug resistance Acinetobacter baumanii to provide a new scheme for the phage therapy of multiple drug resistance Acinetobacter baumanii infection.Methods Multiple drug resistance Acinetobacter baumanii isolated from hospitals in Shanghai served as the host bacteria.The bacteriophages were isolated from different environments.The morphology and size of bacteriophages were observed by electron microscope with negative staining.The influence of pH and temperature on its stability was analyzed.The phage DNA was extracted and sequenced.The related gene function was analyzedResults A virulent phage named D218 of drug-resistance Acinetobacter baumanii was isolated from the effluent sewerage of hospital.The electron microscopy observation showed that it is a long-tail phage of polyhedron symmetric structure,with a diameter of about 65nm,the tail length of about 100nm and width of about 15 nm.The phage kept high activity at pH 3-11 and temperature 4-50 ℃.The genome size is 102449bp with GC content of 37.2%.It encodes 167 ORFs.Conclusion A new virulent phage D218 of drug-resistance Acinetobacter baumanii is isolated,which is stable to pH and temperature,which increases the new data for its application in hospital infection prevention and control,and the phage library setup.
