Analysis on HLA B27 results detected by real-time fluorescence PCR method and flow cytometry
10.3969/j.issn.1673-4130.2017.07.009
- VernacularTitle:实时荧光PCR法和流式细胞术检测HLA-B27的结果分析
- Author:
Ali YE
;
Yaling DONG
;
Lingjun KONG
;
Rui ZHANG
;
Jie YI
- Keywords:
HLA-B27;
real-time PCR;
flow cytometry;
spondylitis
- From:
International Journal of Laboratory Medicine
2017;38(7):892-894
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the clinical application value of the real-time fluorescence PCR method and flow cytometry method for detecting human leukocyte antigen B27(HLA-B27).Methods Blood HLA-B27 level in 225 patients with suspected ankylosing spondylitis was detected by using real-time fluorescence PCR method and flow cytometry method.The detection results were compared and analyzed between the two methods.Results The results of 95.1% sample were identical detected by two methods without statistically significant difference(P>0.05).Taken the results of flow cytometry as reference, the sensitivity of real-time fluorescence PCR for detecting HLA-B27 was 94%, the specificity was 96%.Gene sequencing was performed if results of a sample detected by two methods were different, which was identical with the result detected by real-time fluorescence PCR.Conclusion Both methods for detecting HLA-B27 all have high sensitivity and specificity.Real-time fluorescence PCR method is more superior to the flow cytometry method in the results accuracy.
