Effects of SLeX on invasion and migration of HepG2 cells
10.3969/j.issn.1000-4718.2017.04.018
- VernacularTitle:SLeX对肝癌HepG2细胞迁移和侵袭的影响
- Author:
Wenjuan WU
;
Can CUI
;
Pinfang KANG
;
Yurong SHI
;
Yinghua GENG
- Keywords:
Sialyl Lewis X;
Hepatocellular carcinoma;
HepG2 cells;
Cell migration;
Cell invasion
- From:
Chinese Journal of Pathophysiology
2017;33(4):688-693
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression and effects of sialyl Lewis X (SLeX) on the invasion and migration of human hepatocellular carcinoma HepG2 cells.METHODS: The expression of α1,3-fucosyltransferase VII (FUT7) in HepG2 cells and L-02 cells was detected by RT-qPCR and Western blot.The SLeX expression in HepG2 cells and L-02 cells was determined by Western blot and immunocytochemical staining.The invasion and migration abilities of the treated cells were evaluated by Transwell assay.RESULTS: The expression of FUT7 and SLeX in the HepG2 cells, but not in the L-02 cells, was observed.The invasion rates of the HepG2 cells treated with SLeX monoclonal antibody at 0.05, 0.5 and 5 mg/L were significantly decreased as compared with control group (P<0.05).The migration ability of the HepG2 cells treated with SLeX monoclonal antibody at 0.05, 0.5 and 5 mg/L was also significantly reduced as compared with control group (P<0.05).The invasion rate and migratory cell number were significantly different between any 2 groups in the HepG2 cells treated with SLeX monoclonal antibody at 0.05, 0.5 and 5 mg/L (P<0.05).CONCLUSION: HepG2 cells express SLeX.SLeX is closely related to the migration and invasion abilities of the HepG2 cells.
