Retrospective analysis of external quality assessment results for hemoglobin measurement in Guangxi Province during 2012 to 2016
10.13602/j.cnki.jcls.2017.02.17
- VernacularTitle:2012至2016年广西血红蛋白组分室间质量评价结果的回顾性分析
- Author:
Juan TANG
;
Weiya ZHOU
;
Xiangyang ZHOU
;
Xiaochun LIU
;
Yi HE
- Keywords:
thalassemia;
haemoglobin A2;
haemoglobin F;
external quality assessment
- From:
Chinese Journal of Clinical Laboratory Science
2017;35(2):142-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the detection competence of HbA2 and HbF in Guangxi medical laboratories.Methods The external quality assessment(EQA) of HbA2 and HbF was conducted twice a year and five samples was detected each time during 2012 to 2016.The laboratories participated in EQA completed the samples' detection and submitted the detection results at specified time according to the requirements of EQA.The distribution of each detection system,the qualification rate of each laboratory,the variation degrees of each detection system and each detection method,and the variations of results for different levels of quality control(QC) materials during 5 years were analyzed based on the returned results.Results The application of high performance liquid chromatography (HPLC) and capillary electrophoresis(CE) increased year by year and their usage rates in 2016 reached up to 46.1％ (82/178) and 18.0％ (32/178),respectively.The qualification rates of HbA2 and HbF increased from 51.5％ (34/66) and 60.6％ (40/66) in 2012 to 93.3％ (166/178) and 92.1％ (164/178) in 2016,respectively.The average coefficient of variation(CV) of each detection system decreased year by year.There were good CVs for the results of high,medium and low levels of HbA2 QC materials detected by the Bio-Rad Variant Ⅱ and Sebia CAPILLARYS 2 systems,and they were less than 6.0％.HPLC and CE could quantitatively detect the HbA2 and HbF levels,and their total detection competence was superior to that of agarose gel electrophoresis.Conclusion EQA can assess the abihty of one laboratory detecting HbA2 and HbF,and quantitatively analyze the levels of HbA2 and HbF,which may provide the quality assurance and data support for the screening and prevention of thalassemia.
