Research progresses of applying MALDI-TOF mass spectrometry in the detection of β-lactamase
10.3969/j.issn.1674-8115.2017.04.029
- VernacularTitle:基质辅助激光解析电离飞行时间质谱检测β-内酰胺酶的研究进展
- Author:
Jiajia YU
;
Jing YU
;
Ying LIU
- Keywords:
matrix assisted laser desorption ionization time of flight mass spectrometry;
β-lactamase;
hydrolysis test;
single nucleotide polymorphisms
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2017;37(4):566-570
- CountryChina
- Language:Chinese
-
Abstract:
Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is an emerging tool for detecting microorganisms. It can be used not only for rapid identification of microorganisms, but also for the research of resistance mechanisms. Producing β-lactamase is the main mechanism for resistance of β-lactam antibiotics in Gram-negative bacilli. Currently using MALDI-TOF MS for rapid detection of bacterialβ-lactamase has been widely reported, including detection of β-lactam hydrolysis activity of β-lactamase, direct detection of β-lactamase molecule, peptide and relevant proteins, and detection of the single nucleotide polymorphisms (SNPs) of β-lactamase gene. This paper reviews current application of MALDI-TOF MS to the detection of β-lactamase based on the latest research findings.
