Establishment of metastatic colorectal cancer patient-derived xenografts models by image-guided biopsy
10.19401/j.cnki.1007-3639.2017.04.006
- VernacularTitle:基于穿刺活检的复发转移性结直肠癌患者源性异种移植模型的建立
- Author:
Han MA
;
Ying WANG
;
Guangzhi WANG
;
Lichao XU
;
Xinhong HE
;
Wentao LI
- Keywords:
Biopsy;
Metastatic colorectal cancer;
Patient-derived xenografts models
- From:
China Oncology
2017;27(4):276-280
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose: Current colorectal cancer patient-derived xenografts (PDXs) models were established by samples taken during surgery. However, metastatic colorectal cancer (mCRC) patients have less surgical opportunities, and it was difcult to obtain enough tumor fragment. The aim of the present study was to es-tablish mCRC PDXs by image-guided biopsy. Methods: A total of 12 patients with colorectal cancer who underwent surgery were included. All patients had recurrent lesions or metastatic lesions needed to be histologically confirmed, and none of them had contraindication to biopsy. Tumor tissues not required for clinical diagnosis were used to establish mCRC PDXs. Results: Seven PDXs grew sufficiently for transfer into mice. The success rate was 77.8%. Conclusion:The PDXs established by image-guided biopsy had the advantage of convenient operation, good reproducibility, high achievement ratio, short experimental periodicity and reliably retain specific genetic and morphological features of the primary patient tumors.
