Optimization of Extraction Technology of Compound Rhodiolae crenulatae Granule by Multi-index Ortho-gonal Test
10.6039/j.issn.1001-0408.2017.13.29
- VernacularTitle:多指标正交试验优化复方红景天颗粒的提取工艺
- Author:
Qinghuan LIU
;
Hong WANG
;
Ting ZHANG
;
Yan ZHANG
;
Wentong WANG
;
Zunwei TAO
- Keywords:
Compound Rhodiolae crenulatae granule;
Extraction technology;
Efficacy index;
Mice;
Salidroside;
Ferulic acid;
Orthogonal test
- From:
China Pharmacy
2017;28(13):1830-1833
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the extraction technology of Compound Rhodiolae crenulatae granule. METHODS:The pharmacodynamic index of learning and memory ability was used to screen the water extraction,water extraction and alcohol pre-cipitation and alcohol extraction methods for Compound R. crenulatae granule in mice by Y-type water maze test. Using the compre-hensive scores of salidroside,ferulic acid extraction amount and extraction yield as indexes,orthogonal test was used to optimize the ethanol volume fraction,extraction time,solvent amount and extraction times,and verification test was conducted. RESULTS:Products by alcohol extraction can significantly improve learning and memory ability in the 3 methods;the optimal extraction tech-nology was confirmed to 8-fold 70% ethanol reflux extracting for 3 times,1.0 h each time. In verification test,average extraction amount of salidroside and ferulic acid were 4.884 mg/g and 1.669 mg/g,and average extraction yield was 33.52%(all RSD<2%, n=3). CONCLUSIONS:The optimized extraction technology is stable,reliable,simple and feasible,which can be used for indus-trial extraction of Compound R. crenulatae granule.
