Study on the Extraction Technology of Phenolic Acids from Anemone altica
10.6039/j.issn.1001-0408.2017.13.34
- VernacularTitle:九节菖蒲中酚酸类成分的提取工艺研究
- Author:
Xiaodong YANG
;
Fubing LI
;
Jiao MEI
;
Hongjia YUAN
;
Xingwen LIU
;
Bo LI
- Keywords:
Anemone altica;
Phenolic acids;
Mono-ferulyl tartaric acid;
Ferulic acid;
Content determination;
Extraction tech-nology;
HPLC;
Orthogonal test
- From:
China Pharmacy
2017;28(13):1848-1851
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the content of phenolic acids from Anemone altica,and optimize its extraction technolo-gy. METHODS:HPLC was used to determine the contents of mono-ferulyl tartaric acid and ferulic acid from A. altica;using the total contents of 2 index components as index,volume fraction of extraction solvent,extraction solvent volume,extraction times and extraction time as factors,orthogonal test was used to optimize extraction technology,and verification test was conducted. RE-SULTS:The contents of mono-ferulyl tartaric acid and ferulic acid were 0.059%,0.0025%,respectively;the optimal extraction technology was as follow as 30% ethanol 600 mL added to 20 g medicinal material,extracted twice,90 min every time. In verifi-cation test,the average contents of 2 components in extract were 0.2971%(RSD=3.64%,n=3),0.0041%(RSD=5.11%,n=3). CONCLUSIONS:A method for contents determination of mono-ferulyl tartaric acid and ferulic acid from A. altica is estab-lished;optimized extraction technology is stable and feasible.
