Study of Efficiency of Coupling Peptides with Gold Nanoparticles
10.11895/j.issn.0253-3820.160799
- VernacularTitle:多肽在金纳米粒子表面偶联反应效率研究
- Author:
Qiang ZHANG
;
Ruixue LI
;
Xin CHEN
;
Xingxing HE
;
Ailing HAN
;
Guozhen FANG
;
Jifeng LIU
;
Shuo WANG
- Keywords:
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride;
N-Hydroxysuccinimide;
Gold nanoparticles;
Peptides
- From:
Chinese Journal of Analytical Chemistry
2017;45(5):662-667
- CountryChina
- Language:Chinese
-
Abstract:
Fluorescence spectroscopy was used to investigate the efficiency of coupling peptides to gold nanoparticles via 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride-N-hydroxysuccinimide (EDC-NHS).The experiment conditions including buffer solution, pH value and concentrations of buffer solution, concentrations of NHS and EDC, concentration ratios of NHS to EDC, and coupling reaction time on the coupling efficiency were investigated and optimized.The experimental results indicated that the optimized experimental conditions were as follows: 25 mmol/L HEPES buffer solution, pH 7.0;2∶1 of concentration ratio of NHS to EDC, 0.4 mol/L NHS, 0.2 mol/L EDC, and coupling reaction time of 24 h.This study may provide references for the relative research involving coupling peptide or protein with gold nanoparticles
