Preparation of Carboxybetaine-based Hydrophilic Interaction Chromatographic Column and Its Performance Exploration
10.11895/j.issn.0253-3820.160787
- VernacularTitle:羧基甜菜碱型亲水作用色谱柱的制备及性能研究
- Author:
Yuyang CHANG
;
Xiaojun DAI
;
Bolin GONG
- Keywords:
Hydrophilic interaction chromatography;
Stationary phase;
Carboxybetaine;
Atom transfer radical polymerization
- From:
Chinese Journal of Analytical Chemistry
2017;45(5):734-740
- CountryChina
- Language:Chinese
-
Abstract:
2-(Dimethylamino)ethyl methacrylate and sodium monochloroacetate were employed to synthesize [2-(Methacryloyloxy)ethyl] dimethyl ammonium acetate (CBMA) functional monomer.CBMA was grafted on the surface of silica by surface initiated atom transfer radical polymerization (SI-ATRP) to obtain silica-CBMA hydrophilic interaction stationary phase.Three silica-CBMA stationary phases with different grafted density of CBMA monomer were synthesized in SI-ATRP progress by changing the concentration of CBMA under the same conditions.The ability to separate organic acid compounds of the synthesized silica-CBMA stationary phases was evaluated under different conditions, including effects of pH value, salt concentration and content of water of mobile phase on retention of solutes.The results showed that the stationary phases could effectively separate organic acid compounds in HILIC mode, which followed a mixed mode of chromatography of ion exchange and hydrophilic interaction.The retention of solutes decreased with the increases of salt concentration of mobile phase, which consistent with the characteristics of ion exchange;the pH value of mobile phase had significant influence on ionization of the stationary phase and solutes, i.e., the retention of solutes increased as the increasing of pH value of mobile phase.However, the retention of solutes decreased with the increasing of the content of water in mobile phase, which was the typical characteristic of HILIC.The method of hydrophilic interaction chromatography combined with silica-CBMA stationary phases could conveniently determinate the content of vitamin C and rutin in rutin tablets, providing a new method for the separation and determination of strong polar samples.
