Simultaneous Determination of Seven Kinds of Fluorescent White Agents in Mushroom by QuEChERS-UltraPerformance Liquid Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.160866
- VernacularTitle:QuEChERS-超高效液相色谱-串联质谱法测定食用菌中7种荧光增白剂
- Author:
Xianchen ZHANG
;
Rong LI
;
Yanping ZHOU
;
Yanna BO
;
Jingli WANG
;
Yiguang HU
- Keywords:
Ultra performance liquid chromatography-tandem mass spectrometry;
Fluorescent white agents;
Mushroom
- From:
Chinese Journal of Analytical Chemistry
2017;45(5):777-783
- CountryChina
- Language:Chinese
-
Abstract:
A method based on QuEChERS coupled with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the determination of seven kinds of fluorescent white agent residues in mushroom.After mixing with 10 mL of water, the sample was extracted with acidified acetonitrile, cleaned up by C18, primary secondary amine (PSA) and MgSO4.The separation of seven kinds of fluorescent white agents was performed on a C18 column with a gradient elution of acetonitrile and 0.1% acidified water as mobile phase.The target compounds were detected by electrospray ionization mass spectrometry in positive ion mode with multi reaction monitoring (MRM).Under the optimum conditions, the method had good linear relationship in the determination of the seven kinds of fluorescent white agents in a certain concentration range, with correlation coefficients greater than 0.991.Moreover, the limits of detection (S/N=3) were 0.05-0.4 μg/kg, the limits of quantitation (S/N=10) were 0.2-1.3 μg/kg, and the average recoveries for seven kinds of fluorescent white agent residues in msushroom were 70.1%-109.2%.In comparison with previous methods, the new procedure has characteristics of simple sample preparation and higher sensitivity.
