Determination of Polysaccharide Content in Dried Peel of Seeding Watermelon and Its Mechanism of Lowering Blood Glucose
10.3870/j.issn.1004-0781.2017.05.004
- VernacularTitle:打瓜干皮中多糖的含量测定及降血糖作用机制
- Author:
Yihui ZHANG
;
Liping TIAN
;
Lin XUE
;
Jing SHI
;
Rui HE
- Keywords:
Polysaccharides;
dried peel of seeding watermelon;
Content determination;
α-glucosidase;
Mechanism of lowering blood glucose
- From:
Herald of Medicine
2017;36(5):477-480
- CountryChina
- Language:Chinese
-
Abstract:
Objective Determination of polysaccharide content in dried peel seeding watermelon, and its mechanism of lowering blood glucose.Methods The content of polysaccharides in dried peel of seeding watermelon was determined by the method of phenol-sulfuric acid.Mice were given starch and sucrose load,and the mouse blood glucose was examined.The inhibitory activities of seeding watermelon against α-glucosidase were tested by the colorimetry of pNPG.Results The calibration of polysaccharide was A=0.066 4 C+0.022 6, R2=0.999 5, the content of polysaccharide in dried peel of seeding watermelon was 4.45% (n=10,RSD=1.80%);50,100 and 150 mg·kg-1 of polysaccharide could significantly reduce the starch load in mice blood glucose(P<0.01);100 and 150 mg·kg-1 of polysaccharide significantly reduced sucrose load blood glucose(P<0.01);polysaccharide concentration in 18 mg·mL-1 could significantly inhibit the activity of α-glucosidase as the inhibitory rate was (73.19±3.45)% (n=10).Conclusion Seeding watermelon polysaccharide has effect on lowering blood glucose in starch and sucrose load mice, and inhibits α-glucosidase significantly.
