Valuation Traceability of Reference Extract of Total Lactones from Ginkgo Leaf for Quantitative Analysis
10.3870/j.issn.1004-0781.2017.05.021
- VernacularTitle:定量用银杏叶总内酯对照提取物的量值溯源
- Author:
Qinwei HUANG
;
Bei SHI
;
Ruwei WANG
;
Jianbiao YAO
;
Ming ZHU
;
Cheng ZHENG
- Keywords:
Total lactones from Ginkgo leaf;
Reference extract;
Assigned traceability value
- From:
Herald of Medicine
2017;36(5):554-557
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the phmaceutical reference materials of total lactones extract from ginkgo leaf for quantitative analysis.Methods A HPLC determination method was developed to investigate the uniformity and stability of the reference extract of ginkgo leaf total lactones using with bilobalide, ginkgolide A, ginkgolide B and ginkgolide C as the indexes.Three laboratories participated in the collaborative calibration test.Results The four components in reference extract had good uniformity and stability with RSD less than 2.0%;the marked values of the four components had been determined through statistical data analysis which provided by assigned traceability values.The values of bilobalide, ginkgolide A, ginkgolide B, and ginkgolide C were 39.54%, 29.03%, 15.96% and 11.69%, respectively.Conclusion The reference extract of ginkgo leaf total lactones can be prepared for quality control in future quantitative analysis.
