Methods for identification of citrullinated proteins in tissue cells
10.16571/j.cnki.1008-8199.2017.04.018
- VernacularTitle:组织细胞内瓜氨酸化蛋白的鉴定方法
- Author:
Xiaolong GUAN
;
Ying ZHOU
- Keywords:
Citrullination;
Mass spectrometry;
Rheumatoid arthritis
- From:
Journal of Medical Postgraduates
2017;30(4):421-426
- CountryChina
- Language:Chinese
-
Abstract:
Citrullination is a post-translational modification of arginine caused by peptidyl arginine deiminase in the presence of calcium ions.Up-regulation of citrullination plays a significant role in the progress of numerous disorders,and it has important physiologic and pathological significance.High-throughput and quantitative methods with high specificity and sensitivity are still needed to identify the citrullinated sites in tissue cells.With the development of chemical derivatization and mass spectrometry, new rapid and accurate proteomics technology still has a good prospect of development and application.This article aimed to provide new ideas and directions to identify the citrullinated sites in the complex biological samples by introducing strategies available to identify citrullinated peptides, such as color development reagent analysis, immunodetection and mass spectrometry.
