Expression of indoleamine 2, 3-dioxygenase in condyloma acuminatum lesions
10.3760/cma.j.issn.0412-4030.2017.05.006
- VernacularTitle:吲哚胺2,3-双加氧酶在尖锐湿疣皮损中表达的研究
- Author:
Zhen XIE
;
Yuanhan CHEN
;
Siyu WANG
;
Huiying WAN
;
Hua LEI
;
Ge YANG
;
Zhaochun LIN
- Keywords:
Condylomata acuminata;
Indoleamine-pyrrole 2;
3;
-dioxygenase;
Langerhans cells;
Immunohistochemistry;
Fluoroimmunoassay
- From:
Chinese Journal of Dermatology
2017;50(5):337-340
- CountryChina
- Language:Chinese
-
Abstract:
Objective To measure the expression of indoleamine 2, 3-dioxygenase(IDO)in condy-loma acuminatum (CA) lesions, and to evaluate its ability to locally metabolize tryptophan. Methods Immunohistochemical study was performed to observe the protein expression of IDO in skin lesions of patients with CA, and count the number of IDO-positive cells. Immunofluorescence assay was conducted to estimate the relationship between IDO-positive cells and dendritic cells. Epidermal cells and keratinocytes were isolated from warts of 30 patients with CA and prepuces of 11 healthy controls respectively, and both in vitro incubated with tryptophan solution for 4 hours. Then, high-performance liquid chromatography (HPLC)was performed to detect the level of tryptophan metabolite, kynurenine, in the culture supernatant of the above cells, which could reflect the ability of epidermal cells to metabolize tryptophan. Results Rare IDO-positive cells were found in the normal skin, but a lot of IDO-positive cells gathered in the epidermis of the wart tissues. The IDO-positive cell/total cell ratio was significantly higher in the wart tissues than in the normal skin(48.3%± 15.4%vs. 5.2%± 2.4%, P<0.05). The fluorescence signals of IDO-positive cells and CD1a-positive Langerhans cells were not overlapped with each other, suggesting that IDO-positive cells were derived from epidermal cells of the wart tissues. Compared with the keratinocytes from the healthy skin, the epidermal cells from warts had a stronger ability to metabolize tryptophan in vitro. Conclusion A large number of IDO-positive cells exist in CA warts, and may be involved in occurrence of CA.
