Acridine orange staining for fast identification ofthe spermatogenic cells during STA-PUT velocity sedimentation
- VernacularTitle:一种利用吖啶橙染色快速鉴定重力密度梯度沉降法中生精细胞类型的方法
- Author:
Qing ZHAO
;
Ruiqin HAN
;
Linfang WANG
;
Wei SONG
- Keywords:
STA-PUT velocity sedimentation;
pachytene spermatocytes;
round spermatids;
elongating and condensing spermatids;
acridine orange staining
- From:
Basic & Clinical Medicine
2017;37(5):591-595
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a fast and accurate technique of identifying the pachytene spermatocytes,round spermatids and elongating and condensing spermatids during STA-PUT velocity sedimentation.Methods Using STA-PUT velocity sedimentation method to isolate the pachytene spermatocytes, round spermatids and elongating and condensing spermatids from mouse testes.To determine the cell populations` distribution,each tube of cell fraction was then partially transfered to the 96 plate well,and each well was added with acridine orange dye.Then each well was analyzed using fluorescence microscopy.Results Three types of spermatogenic cells can be identified quickly and accurately by it`s specific cytoplasm/nucleus character using the acridine orange dye staining under fluorescence detection.Conclusions A rubust method to quickly and accurately determine the pachytene spermatocytes,round spermatids and elongating and condensing spermatids during STA-PUT velocity sedimentation is successfully developed.
