Insulin resistance and low leveladiponectin co-promote cardiac remodeling in mice
- VernacularTitle:胰岛素抵抗与低水平脂联素协同促小鼠心室重塑
- Author:
Lu XU
;
Yajuan LIU
;
Qing ZHANG
;
Na ZHANG
;
Yongkang REN
;
Ruiying YANG
- Keywords:
insulin resistance;
adiponectin;
cardiac remodeling
- From:
Basic & Clinical Medicine
2017;37(5):625-629
- CountryChina
- Language:Chinese
-
Abstract:
Objective The study the effect on cardiac remodeling induced by low level adiponectin(APN)and insulin resistance(IR).Methods 16 wild-type C57 mice were randomly divided into control and IR groups.16 APN gene knockout mice were randomly divided into APNKO and APNKO+IR groups.The IR and APNKO+IR groups were a high-fat feed to induce insulin resistance.After 12 weeks,cholesterol(TC),triglycerides(TG),fasting plasma glucose(FPG), fasting insulin(FINS) were measured.HOMA-IR,HWI and LVWI were calculated.The left ventricular myocardial were examined by microscopy to observe the degree of myocardial fibrosis,and the protein expression of matrix metalloproteinase-9(MMP-9) and APN in myocardial were detected with western blot.ResultsLow level APN and IR increased heart weight, left ventricular weight, HWI, LVWI(P<0.05).There were interaction between IR and low level APN on the TC,TG, FPG, FINS, and the index related myocardial fibrosis(P<0.05), and they were positive effect.There was also interaction effect between IR and low level APN on myocardial APN expression, but it was the negative effects (P<0.05).Conclusions Both low level adiponectin and insulin resistance could lead to cardiac remodeling synergistically.
