Progress of Chemical Derivatization in Analysis of Endogenous Anabolic Steroids in Bio-matrices
10.3870/j.issn.1004-0781.2017.05.017
- VernacularTitle:衍生化技术用于生物基质中类固醇类激素分析的研究进展
- Author:
Qiuju HU
;
Jianyun YANG
;
Bingkun XIAO
;
Rongqing HUANG
- Keywords:
Endogenous anabolic steroids;
Derivatization;
Instrument analysis
- From:
Herald of Medicine
2017;36(5):532-537
- CountryChina
- Language:Chinese
-
Abstract:
The content of endogenous anabolic steroids is extremely low in biological matrices.Its chemical structure contains polar functional groups such as hydroxyl and carbonyl, which limits their applicability in GC-MS.The lack of ionized groups in the chemical structure leads to poor sensitivity in LC-MS, which plays a significant role in various physiological activities analysis.It is an effective way to enhance the response of mass spectrometer by modifying the chemical structure of endogenous anabolic steroids through derivatization technology.This review summarizes various derivatization reagents and corresponding derivatization processes of endogenous anabolic steroids analysis in the methods based on different testing instruments and methodologies.The advantage and disadvantage of all kinds of derivatiaztion methods and the prospect of the endogenous anabolic steroids derivatiaztion techniques are also discussed.
