The Effect of TNF-α Blocker HL036337 and Its Best Concentration to Inhibit Dry Eye Inflammation.
10.3341/kjo.2016.30.4.302
- Author:
Wungrak CHOI
1
;
Hyemi NOH
;
Areum YEO
;
Hanmil JANG
;
Hyea Kyung AHN
;
Yeon Jung SONG
;
Hyung Keun LEE
Author Information
1. Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. shadik@yuhs.ac
- Publication Type:Original Article
- Keywords:
Corneal erosion;
Dry eye;
HL036337;
Inflammation;
Tumor necrosis factor-alpha
- MeSH:
Animals;
Cornea/diagnostic imaging;
Disease Models, Animal;
Dose-Response Relationship, Drug;
Dry Eye Syndromes/diagnosis/*drug therapy;
Female;
Mice;
Mice, Inbred C57BL;
Microscopy, Acoustic;
Ophthalmic Solutions/administration & dosage;
Tumor Necrosis Factor-alpha/*antagonists & inhibitors
- From:Korean Journal of Ophthalmology
2016;30(4):302-308
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Dry eye syndrome is commonly thought of as an inflammatory disease, and we have previously presented data showing the effectiveness of topical TNF-α blocker agents for the treatment of this condition. The purpose of this study was to investigate the effectiveness of the TNF-α blocking agent HL036337 compared to cyclosporine A for the treatment of dry eye induced inflammation in order to establish whether HL036337 represents a more effective method for suppressing inflammation. The efficacy of HL036337 and cyclosporine A was determined using an experimental murine dry eye model. METHODS: The TNF-α blocker HL036337 is a modified form of TNF receptor I. Using dry eye induced C57BL/6 mice (n = 45), corneal erosion was measured at day 4 and 7 after topical treatment with cyclosporine A or HL036337. To determine the effective treatment dose, 0.25, 0.5, 1, 2.5, and 5 mg/mL of HL036337 were topically administered twice per day to dry eye induced murine corneas for 1 week. RESULTS: The optimal concentration of the TNF-α blocker HL036337 for treatment of dry eye induced corneal erosion was determined to be 1 mg/mL. Dry eye induced corneal erosion was improved after 1 week with topically applied cyclosporine A and HL036337 at 1 mg/mL. CONCLUSIONS: HL036337 administered topically at 1 mg/mL effectively improved corneal erosion induced by dry eye. This finding may also suggest that inhibition of TNF-α can improve dry eye syndrome.
