The changes on expression of IL-1βand IL-13 mRNA in lung tissue and serum after drowning rats
10.13618/j.issn.1001-5728.2017.02.006
- VernacularTitle:大鼠溺死后肺组织与血清IL-1β、IL-13mRNA表达变化
- Author:
Meiling JIANG
;
Wenping GUO
;
Jinfang ZHAO
;
Junfeng LU
;
Ze MEI
;
Jiajia LIU
;
Xinhua LIANG
- Keywords:
forensic pathology;
drowning;
TaqMan probe method;
cytokine;
IL-1β;
IL-13
- From:
Chinese Journal of Forensic Medicine
2017;32(2):137-140
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the change in IL-1β,IL-13mRNA expression in drowning rat lungs and serum,so as to investigate the significance of IL-1β and IL-13 mechanism in the development of drowning.Methods SD rats were randomly divided into control group,drowning group.Then using TaqMan probe method to determine the expression of IL-1β and IL-13 mRNA in Right lower lobe of lung tissue and the serum of right ventricle,which were extracted respectively from each group of rats.Results (1) The lung tissue morphological changes:Typical appearance signs and anatomy of drowning group meet ante-mortem drowning feature.(2) The expression of IL-1β,IL-13 in lung tissue:compared with the control group,the expression of IL-1β and IL-13 were slightly decreased,which has no statistical significance.(3) The expression of IL-1β and IL-13 in serum:compared with the control group,the expression of IL-1β and IL-13 were significant increased,both of which has statistical significance.Conclusion (1)The expression of IL-1β and IL-13 were decreased in lung tissue may be due to drowned rats present compensatory anti-inflammatory response syndrome which causes immune incompetent performance.(2) The expression of IL-1β and IL-13 were significant increased in serum may be relate to drown stress and drowning associated acute lung injury after traumatic stress.
