The expression and clinical significance of miR-345 in non-small cell lung cancer
10.11904/j.issn.1002-3070.2017.02.005
- VernacularTitle:miR-345在非小细胞肺癌中的表达及临床意义
- Author:
Haifeng HU
;
Xiemin FENG
;
Jian QIAO
;
Wei DUAN
;
Ningning LIU
;
Xiaodong SUN
- Keywords:
Biomarker;
miR-345;
Non-small cell lung cancer;
Prognosis
- From:Practical Oncology Journal
2017;31(2):117-122
- CountryChina
- Language:Chinese
-
Abstract:
Objective The objective of this study was to investigate the miR-345 expression level in non-small cell lung cancer(NSCLC)tissue and its potential clinical significance.Methods Real-time PCR was conducted to evaluate the expression of miR-345 in NSCLC tissues in 98 samples and cell lines.Then the association between tissue miR-345 expression level and clinical outcomes was further analyzed.Results The expression level of miR-345 was significantly decreased in NSCLC tissues and cell lines when compared to the controls(P<0.05).miR-345 expression level in NSCLS tissues was associated with various clinicopathological parameters including LN metastasis(P<0.001),distant metastasis(P=0.028),TNM stage(P=0.004)and grade(P=0.011).In addition,the NSCLC patients in the low miR-345 expression group showed significantly shorter overall survival time than that in the high miR-345expression group(P=0.021).Multivariate analysis showed that the expression of miR-345 was an independent risk factor for NSCLC(HR=3.897,95% CI:2.263~10.440;P=0.012).Conclusion The expression level of miR-345 was decreased in NSCLC tissues as well as cell lines compared with normal controls.Low miR-345 expression in tissues was associated with progression and poor prognosis of NSCLC.These results indicate that miR-345 may be a novel prognostic marker in NSCLC.
