Effect of bone marrow mesenchymal stem cell transplantation on silicosis fibrosis in rats
10.3969/j.issn.1671-8348.2017.12.001
- VernacularTitle:骨髓间充质干细胞移植对矽肺大鼠肺纤维化的作用
- Author:
Zhijun CHEN
;
Huixia HUANG
;
Ying XIE
;
Donghong LI
;
Zhimin LI
;
Jianjie ZHANG
- Keywords:
silicosis;
pulmonary fibrosis;
mesenchymal stem cells;
hydroxyproline;
rats;
Sprague-Dawley
- From:
Chongqing Medicine
2017;46(12):1585-1587
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of bone mesenchymal stem cells(BMSCs)transplantation on pulmonary tissue fibrosis in silicosis rats.Methods Forty-two healthy female SD rats were randomly divided into 4 groups:group A,B,C and D.The group A,B and C received intratracheal injection of silica crystals(SiO2)to establish silicosis mouse models,while the group D received the infusion of physiological saline.BMSCs were injected by the tail vein at 6 h after modeling or on 42 d in the group B and C.Six rats in each of group A,B,and D were sacrificed on 14 d,and other 6 rats in each group were sacrificed on 56 day.The morphological changes of the lung tissues were observed by HE staining;the content of hydroxyproline(Hyp)in the lung tissue homogenate was detected by ELISA.Results The lung tissue pathological changes in the group A were dominated by acute inflammatory response on 14 d after model construction,and fibrosis reaction on 56 d;the pathological changes in the group B and C were lighter than those in the group A;while no obvious abnormality was found in the group D.The Hyp level of pulmonary tissue in the group A,B and C was significantly increased compared with the group D(P=0.000);which on 14 d after BMSCs treatment in the group B and C was significantly decreased compared with the group A(P=0.000,P=0.035),and which on 56 d after treatment in the group B was still significantly decreased(P=0.016).Conclusion No matter in the pathological stages of pulmonary alveolitis and fibrosis,BMSCs transplantation may postpone the pathological process of pulmonary fibrosis in silicosis rats.
