β-catenin high expression in atherosclerotic vessel wall caused by rabbit carotid artery cannula
10.3969/j.issn.1671-8348.2017.12.002
- VernacularTitle:β-catenin在兔颈总动脉套管导致的动脉粥样硬化血管壁中高表达
- Author:
Yuehua LIU
;
Yan SHENG
;
Yingjie TU
;
Xing YANG
;
Gangwei OU
;
Xin SHENG
- Keywords:
carotid artery cannula;
atherosclerosis;
β-catenin;
animal experimentation;
rabbits
- From:
Chongqing Medicine
2017;46(12):1588-1591
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of rabbit carotid artery cannula on atherosclerosis formation and β-catenin expression.Methods Fifteen 2-month-old New Zealand rabbits weighing(2.0±0.2)kg were randomly divided into three groups,high-fat diet group,left common carotid artery cannula group and left common carotid artery cannula plus high-fat diet group,5 cases in each group,and taking the right blood vessels in the left common carotid artery cannula group served as the control group.The animals were sacrificed after 9-week feeding,and the total rabbit carotid artery in each group was taken;the real-time PCR and immunohistochemistry were used to detect β-catenin mRNA and protein expression targeting in rabbit common carotid artery tissue.Results The real-time PCR results showed that the β-catenin mRNA expression in the left common carotid artery cannula group was higher than that in the control group,high fat diet group and the left common carotid artery cannula + high-fat diet group.The immunohistochemistry results showed that,except for the control group,various groups had the β-catenin protein location in the cytoplasma,moreover which in the left carotid artery cannula group and left common carotid artery cannula +high-fat diet group mainly located in the area of intimal hyperplasia.Conclusion β-catenin is highly expressed in the atherosclerotic vessel wall caused by rabbit carotid artery cannula.
