Study of pro-angiogenesis effect of neuropilin-2 on pancreatic neuroendocrine tumors
10.3969/j.issn.1671-8348.2017.12.005
- VernacularTitle:神经纤毛蛋白2促进胰腺神经内分泌肿瘤血管生成作用的研究
- Author:
Xiong XIE
;
Shuang WU
;
Weiwen YANG
;
Yue ZHANG
;
Juanjuan OU
;
Houjie LIANG
;
Xueli PANG
- Keywords:
pancreatic neuroendocrine tumors;
neuropilin-2;
angiogenesis
- From:
Chongqing Medicine
2017;46(12):1599-1601
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role and significance of neuropilin-2(NRP2)for regulating the angiogenesis of pancreatic neuroendocrine tumors(PNETs).Methods The NRP2 expression in pancreatic neuroendocrine tumer BON-1 cell line was intevened.The BON-1 cells cultural supernatants in the control group and interference group were used to treat human umbilical vein endothelial cells(HUVEC).CCK-8 was used to detect the cell proliferation,Transwell was used to detected the cell migration and the tubule formation test was used detect the pro-angiogenesis.Results The CCK-8 detection showed that there was no statistically significant difference in the supernatant treated HUVEC proliferations between the interference group and control group medium(P>0.05):the absorbancy in the control group was 0.35±0.04,while which in the interference group was 0.32±0.04.The Transwell test showed that the invasion ability of HUVEC treated with cultural supernatants in the interference group was weakened compared with the control group,the control group was(203±13)/hole,while the interference group was(100±10)/hole(P＜0.01);the tubule formation test showed that HUVEC tubular formation treated by cultural supernatant in the interference group was decreased,the control group was 40±5,while the interference group was 24±3(P＜0.01).Conclusion Interfering NRP2 expression of BON-1 cells can inhibit the vessel formation ability of co-cultured HUVEC,suggesting that NRP2 may have the pro-angiogenesis effect of PNETs,and may be a potential new target for the treatment of PNETs.
