Cytotoxic effect and mechanism of Aflatoxins B1 on Vero E6 cells
10.3969/j.issn.1673-4130.2017.08.001
- VernacularTitle:黄曲霉毒素B1对非洲绿猴肾细胞的毒性效应及作用机制研究
- Author:
Shan LIU
;
Zhongqiu LUO
;
Xiaoming FAN
;
Hao WU
;
Mingyong LI
;
Hong TAN
- Keywords:
aflatoxins B1;
african green monkey kidney cells;
cell apoptosis;
cytotoxicity
- From:
International Journal of Laboratory Medicine
2017;38(8):1009-1011
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the cytotoxic effects and mechanisms of Aflatoxins B1(AFB1) on the African green monkey kidney cells (Vero E6).Methods After incubation of Vero E6 cells with AFB1,cellular toxicity was assessed by CCK-8 assay and LDH release rate.Annexin V/PI double staining and DAPI staining was used to detected cell apoptosis.Results AFB1 has significant toxic effects on Vero E6 cells.Cell viability was less than 10% when treated by 200 μmol/L AFB1 for 48 h.LDH release rate was increased significantly with the increase of AFB1 concentration.Externalization of cell membrane phosphatidylserine was observed by Annexin V/PI double staining.Nucleus fragmentation was detected by DAPI staining.Conclusion AFB1 exhibits significantly cytotoxic effects on Vero E6 cells by inducing cell apoptosis.
