A report of Vibrio sp.GDLAMI-1210,isolated from Ctenogobius gymnauchen cultured in seawater

VernacularTitle:裸项栉鰕虎鱼感染创伤弧菌初报
Author: Qinfang LUO ; Wenke HUANG ; Wen YUAN ; Jinchun PAN ; Ren HUANG ; Ruiai CHEN
Keywords: Ctenogobius gymnauchen(Bleeker); Vibrio sp; Pathogen
From: Acta Laboratorium Animalis Scientia Sinica 2017;25(2):225-228
CountryChina
Language:Chinese
Abstract: Objective To observe and identify the microorganism isolated from diseased and dead Ctenogobius gymnauchen cultured in seawater near the Daya Bay of south China sea.Methods GDLAMI-1210 strain was isolated from the diseased Ctenogobius gymnauchen(Bleeker).We applied physiological and biochemical characteristics in the bacterial classification.In order to confirm the results,we amplified a 1438 bp sequence of GDLAMI-1210's 16 S rRNA(HM 362434)and compared with other sequence in GenBank,and followed by artificial infection.Results The GDLAMI-1210 strain was Gram-negative and in a shape of short rod with single polar flagellum.The homology analysis and phylogenetic study showed that the 16 S rRNA sequence of GDLAMI-1210 has the highest similarity to Vibrio sp.espec Vibrio vulnificus,showing 99% identity.Conclusions To our knowledge,this is the first report that the causative pathogen,Vibrio sp,leads to the mortality of Ctenogobius gymnauchen(Bleeker).